random hexanucleotide-primer kit (Promega)
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Structured Review
Promega
random hexanucleotide-primer kit
Random Hexanucleotide Primer Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/random+hexanucleotide-primer+kit/random+hexanucleotide+primer+kit/pmc09569327-273-19-25
Average 90 stars, based on 1 article reviews
Random Hexanucleotide Primer Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/random+hexanucleotide-primer+kit/random+hexanucleotide+primer+kit/pmc09569327-273-19-25
Average 90 stars, based on 1 article reviews
random hexanucleotide-primer kit - by Bioz Stars,
2026-10
90/100 stars
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Transformation Assay:Article Title: The role of the Aspergillus nidulans high mobility group B protein HmbA, the orthologue of Saccharomyces cerevisiae Nhp6p Article Snippet: [ 32 P]-dCTP labelled hmbA and 18S rRNA gene-specific DNA molecules were used as gene probes using the random Article Title: A novel-type substrate-selectivity filter and ER-exit determinants in the UapA purine transporter. Article Snippet: 0022-2836/$ see front matter q 2005 E A.V. and S.A. contributed equally Abbreviations used: NAT, nucleo porter; TMS, transmembrane segme reticulum.. E-mail address of the correspond diallina@biol.uoa.gr ‡Nucleobase-Cation Symporter; h ucsd.edu/tcdb/ Wepresent a functional analysis of the last a-helical transmembrane segment (TMS12) of UapA, a uric acid-xanthine/H symporter inAspergillus nidulans and member of the nucleobase-ascorbate transporter (NAT) family.. First, we performed a systematic mutational analysis of residue F528, located in the middle of TMS12, which was known to be critical for UapA specificity. Article Title: Comparative kinetic analysis of AzgA and Fcy21p, prototypes of the two major fungal hypoxanthine-adenine-guanine transporter families. Article Snippet: In fungi, uptake of salvageable purines is carried out by members of two evolutionarily distinct protein families, the PurineRelated Transporters (PRT/NCS1) and the AzgA-like Transporters.. We carried out a comparative kinetic analysis of two prototypes of these transporter families.. The first was Fcy21p, a herein characterized protein of Candida albicans, and the second was AzgA, a transporter of Aspergillus nidulans . Article Title: Regulation of expression and kinetic modeling of substrate interactions of a uracil transporter in Aspergillus nidulans. Article Snippet: Early genetic evidence suggested that A. nidulans possesses at least one uracil transporter.. A gene, named furD, was recently identified by reverse genetics and in silico approaches, and we confirm here that it encodes a high-affinity, high-capacity, uracil transporter.. In this work, we study the regulation of expression of FurD and develop a kinetic model describing transporter-substrate interactions. Article Title: The first transmembrane segment (TMS1) of UapA contains determinants necessary for expression in the plasma membrane and purine transport. Article Snippet: UapA, a member of the NAT/NCS2 family, is a high affinity, high capacity, uric acid-xanthine/H symporter in Aspergillus nidulans .. Determinants critical for substrate binding and transport lie in a highly conserved signature motif downstream from TMS8 and within TMS12.. Here we examine the role of TMS1 in UapA biogenesis and function. Article Title: Role of High Mobility Group B protein HmbA, orthologue of yeast Nhp6p in Aspergillus nidulans Article Snippet: [ 32 P]-dCTP labelled hmbA and 18S rRNA gene-specific DNA molecules were used as gene probes using the random Article Title: Comparative substrate recognition by bacterial and fungal purine transporters of the NAT/NCS2 family. Article Snippet: We compared the interactions of purines and purine analogues with representative fungal and bacterial members of the widespread Nucleobase-Ascorbate Transporter (NAT) family.. These are: UapA, a well-studied xanthine-uric acid transporter of A. nidulans, Xut1, a novel transporter from C. albicans, described for the first time in this work, and YgfO, a recently characterized xanthine transporter from E. coli.. Using transport inhibition experiments with 64 different purines and purine-related analogues, we describe a kinetic approach to build models on how NAT proteins interact with their substrates. Southern Blot:Article Title: The role of the Aspergillus nidulans high mobility group B protein HmbA, the orthologue of Saccharomyces cerevisiae Nhp6p Article Snippet: [ 32 P]-dCTP labelled hmbA and 18S rRNA gene-specific DNA molecules were used as gene probes using the random Article Title: A novel-type substrate-selectivity filter and ER-exit determinants in the UapA purine transporter. Article Snippet: 0022-2836/$ see front matter q 2005 E A.V. and S.A. contributed equally Abbreviations used: NAT, nucleo porter; TMS, transmembrane segme reticulum.. E-mail address of the correspond diallina@biol.uoa.gr ‡Nucleobase-Cation Symporter; h ucsd.edu/tcdb/ Wepresent a functional analysis of the last a-helical transmembrane segment (TMS12) of UapA, a uric acid-xanthine/H symporter inAspergillus nidulans and member of the nucleobase-ascorbate transporter (NAT) family.. First, we performed a systematic mutational analysis of residue F528, located in the middle of TMS12, which was known to be critical for UapA specificity. Article Title: Comparative kinetic analysis of AzgA and Fcy21p, prototypes of the two major fungal hypoxanthine-adenine-guanine transporter families. Article Snippet: In fungi, uptake of salvageable purines is carried out by members of two evolutionarily distinct protein families, the PurineRelated Transporters (PRT/NCS1) and the AzgA-like Transporters.. We carried out a comparative kinetic analysis of two prototypes of these transporter families.. The first was Fcy21p, a herein characterized protein of Candida albicans, and the second was AzgA, a transporter of Aspergillus nidulans . Article Title: Regulation of expression and kinetic modeling of substrate interactions of a uracil transporter in Aspergillus nidulans. Article Snippet: Early genetic evidence suggested that A. nidulans possesses at least one uracil transporter.. A gene, named furD, was recently identified by reverse genetics and in silico approaches, and we confirm here that it encodes a high-affinity, high-capacity, uracil transporter.. In this work, we study the regulation of expression of FurD and develop a kinetic model describing transporter-substrate interactions. Article Title: The first transmembrane segment (TMS1) of UapA contains determinants necessary for expression in the plasma membrane and purine transport. Article Snippet: UapA, a member of the NAT/NCS2 family, is a high affinity, high capacity, uric acid-xanthine/H symporter in Aspergillus nidulans .. Determinants critical for substrate binding and transport lie in a highly conserved signature motif downstream from TMS8 and within TMS12.. Here we examine the role of TMS1 in UapA biogenesis and function. Article Title: Role of High Mobility Group B protein HmbA, orthologue of yeast Nhp6p in Aspergillus nidulans Article Snippet: [ 32 P]-dCTP labelled hmbA and 18S rRNA gene-specific DNA molecules were used as gene probes using the random Article Title: Comparative substrate recognition by bacterial and fungal purine transporters of the NAT/NCS2 family. Article Snippet: We compared the interactions of purines and purine analogues with representative fungal and bacterial members of the widespread Nucleobase-Ascorbate Transporter (NAT) family.. These are: UapA, a well-studied xanthine-uric acid transporter of A. nidulans, Xut1, a novel transporter from C. albicans, described for the first time in this work, and YgfO, a recently characterized xanthine transporter from E. coli.. Using transport inhibition experiments with 64 different purines and purine-related analogues, we describe a kinetic approach to build models on how NAT proteins interact with their substrates. Polymerase Chain Reaction:Article Title: The role of the Aspergillus nidulans high mobility group B protein HmbA, the orthologue of Saccharomyces cerevisiae Nhp6p Article Snippet: [ 32 P]-dCTP labelled hmbA and 18S rRNA gene-specific DNA molecules were used as gene probes using the random Article Title: A novel-type substrate-selectivity filter and ER-exit determinants in the UapA purine transporter. Article Snippet: 0022-2836/$ see front matter q 2005 E A.V. and S.A. contributed equally Abbreviations used: NAT, nucleo porter; TMS, transmembrane segme reticulum.. E-mail address of the correspond diallina@biol.uoa.gr ‡Nucleobase-Cation Symporter; h ucsd.edu/tcdb/ Wepresent a functional analysis of the last a-helical transmembrane segment (TMS12) of UapA, a uric acid-xanthine/H symporter inAspergillus nidulans and member of the nucleobase-ascorbate transporter (NAT) family.. First, we performed a systematic mutational analysis of residue F528, located in the middle of TMS12, which was known to be critical for UapA specificity. Article Title: Comparative kinetic analysis of AzgA and Fcy21p, prototypes of the two major fungal hypoxanthine-adenine-guanine transporter families. Article Snippet: In fungi, uptake of salvageable purines is carried out by members of two evolutionarily distinct protein families, the PurineRelated Transporters (PRT/NCS1) and the AzgA-like Transporters.. We carried out a comparative kinetic analysis of two prototypes of these transporter families.. The first was Fcy21p, a herein characterized protein of Candida albicans, and the second was AzgA, a transporter of Aspergillus nidulans . Article Title: Regulation of expression and kinetic modeling of substrate interactions of a uracil transporter in Aspergillus nidulans. Article Snippet: Early genetic evidence suggested that A. nidulans possesses at least one uracil transporter.. A gene, named furD, was recently identified by reverse genetics and in silico approaches, and we confirm here that it encodes a high-affinity, high-capacity, uracil transporter.. In this work, we study the regulation of expression of FurD and develop a kinetic model describing transporter-substrate interactions. Article Title: The first transmembrane segment (TMS1) of UapA contains determinants necessary for expression in the plasma membrane and purine transport. Article Snippet: UapA, a member of the NAT/NCS2 family, is a high affinity, high capacity, uric acid-xanthine/H symporter in Aspergillus nidulans .. Determinants critical for substrate binding and transport lie in a highly conserved signature motif downstream from TMS8 and within TMS12.. Here we examine the role of TMS1 in UapA biogenesis and function. Article Title: Role of High Mobility Group B protein HmbA, orthologue of yeast Nhp6p in Aspergillus nidulans Article Snippet: [ 32 P]-dCTP labelled hmbA and 18S rRNA gene-specific DNA molecules were used as gene probes using the random Article Title: Comparative substrate recognition by bacterial and fungal purine transporters of the NAT/NCS2 family. Article Snippet: We compared the interactions of purines and purine analogues with representative fungal and bacterial members of the widespread Nucleobase-Ascorbate Transporter (NAT) family.. These are: UapA, a well-studied xanthine-uric acid transporter of A. nidulans, Xut1, a novel transporter from C. albicans, described for the first time in this work, and YgfO, a recently characterized xanthine transporter from E. coli.. Using transport inhibition experiments with 64 different purines and purine-related analogues, we describe a kinetic approach to build models on how NAT proteins interact with their substrates. |